PoplarV1, Main, Exploration, bibRecord, 002376

An efficient Agrobacterium-mediated transformation system for poplar.

Identifieur interne : 002376 ( Main/Exploration ); précédent : 002375; suivant : 002377

An efficient Agrobacterium-mediated transformation system for poplar.

Auteurs : Ali Movahedi [République populaire de Chine] ; Jiaxin Zhang [République populaire de Chine] ; Rasoul Amirian [Iran] ; Qiang Zhuge [République populaire de Chine]

Source :

International journal of molecular sciences [ 1422-0067 ] ; 2014.

RBID : pubmed:24933641

Descripteurs français

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Cicer (metabolism), Genetic Vectors (metabolism), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), Regeneration (MeSH), Repressor Proteins (genetics), Repressor Proteins (metabolism), Transformation, Genetic (MeSH).
MESH :
- chemical , genetics : Plant Proteins, Repressor Proteins.
- genetics : Agrobacterium tumefaciens, Populus.
- metabolism : Agrobacterium tumefaciens, Cicer, Genetic Vectors, Plant Leaves, Plant Proteins, Populus, Repressor Proteins.
- Regeneration, Transformation, Genetic.

Abstract

Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybrid clone "Nanlin895" (Populus deltoides×P. euramericana) with Agrobacterium harboring the PBI121:CarNAC6 binary vector, we showed that the transformation efficiency was improved significantly by multiple independent factors, including an Agrobacterium infective suspension with an OD600 of 0.7, an Agrobacterium infection for 120 min, an Agrobacterium infective suspension at a pH of 5.0, an acetosyringone concentration of 200 µM, a cocultivation at 28 °C, a cocultivation for 72 h and a sucrose concentration of 30 g/L in the cocultivation medium. We also showed that preculture of wounded leaf explants for two days increased the regeneration rate. The integration of the desired gene into transgenic poplars was detected using selective medium containing kanamycin, followed by southern blot analysis. The expression of the transgene in the transgenic lines was confirmed by northern blot analysis.

DOI: 10.3390/ijms150610780
PubMed: 24933641
PubMed Central: PMC4100180

Affiliations:

Iran, République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 002129
to stream Main, to step Curation: 002129

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An efficient Agrobacterium-mediated transformation system for poplar.</title>
<author><name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. ali664699@gmail.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Jiaxin" sort="Zhang, Jiaxin" uniqKey="Zhang J" first="Jiaxin" last="Zhang">Jiaxin Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. zhangjiaxin60633@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Amirian, Rasoul" sort="Amirian, Rasoul" uniqKey="Amirian R" first="Rasoul" last="Amirian">Rasoul Amirian</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Genomics, Agricultural Biotechnology Research Institute, Central Region of Iran (ABRICI), Najafabad Road, Isfahan 85135-487, Iran. rasool_amirian@yahoo.com.</nlm:affiliation>
<country xml:lang="fr">Iran</country>
<wicri:regionArea>Department of Genomics, Agricultural Biotechnology Research Institute, Central Region of Iran (ABRICI), Najafabad Road, Isfahan 85135-487</wicri:regionArea>
<wicri:noRegion>Isfahan 85135-487</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. qzhuge@njfu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:24933641</idno>
<idno type="pmid">24933641</idno>
<idno type="doi">10.3390/ijms150610780</idno>
<idno type="pmc">PMC4100180</idno>
<idno type="wicri:Area/Main/Corpus">002129</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002129</idno>
<idno type="wicri:Area/Main/Curation">002129</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002129</idno>
<idno type="wicri:Area/Main/Exploration">002129</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">An efficient Agrobacterium-mediated transformation system for poplar.</title>
<author><name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. ali664699@gmail.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Jiaxin" sort="Zhang, Jiaxin" uniqKey="Zhang J" first="Jiaxin" last="Zhang">Jiaxin Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. zhangjiaxin60633@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Amirian, Rasoul" sort="Amirian, Rasoul" uniqKey="Amirian R" first="Rasoul" last="Amirian">Rasoul Amirian</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Genomics, Agricultural Biotechnology Research Institute, Central Region of Iran (ABRICI), Najafabad Road, Isfahan 85135-487, Iran. rasool_amirian@yahoo.com.</nlm:affiliation>
<country xml:lang="fr">Iran</country>
<wicri:regionArea>Department of Genomics, Agricultural Biotechnology Research Institute, Central Region of Iran (ABRICI), Najafabad Road, Isfahan 85135-487</wicri:regionArea>
<wicri:noRegion>Isfahan 85135-487</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name>
<affiliation wicri:level="1"><nlm:affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. qzhuge@njfu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea>
<wicri:noRegion>Nanjing 210037</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">International journal of molecular sciences</title>
<idno type="eISSN">1422-0067</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term>
<term>Agrobacterium tumefaciens (metabolism)</term>
<term>Cicer (metabolism)</term>
<term>Genetic Vectors (metabolism)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Regeneration (MeSH)</term>
<term>Repressor Proteins (genetics)</term>
<term>Repressor Proteins (metabolism)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term>
<term>Agrobacterium tumefaciens (métabolisme)</term>
<term>Cicer (métabolisme)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines de répression (génétique)</term>
<term>Protéines de répression (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régénération (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
<term>Repressor Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
<term>Populus</term>
<term>Protéines de répression</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Cicer</term>
<term>Genetic Vectors</term>
<term>Plant Leaves</term>
<term>Plant Proteins</term>
<term>Populus</term>
<term>Repressor Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
<term>Cicer</term>
<term>Feuilles de plante</term>
<term>Populus</term>
<term>Protéines de répression</term>
<term>Protéines végétales</term>
<term>Vecteurs génétiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Regeneration</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Régénération</term>
<term>Transformation génétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybrid clone "Nanlin895" (Populus deltoides×P. euramericana) with Agrobacterium harboring the PBI121:CarNAC6 binary vector, we showed that the transformation efficiency was improved significantly by multiple independent factors, including an Agrobacterium infective suspension with an OD600 of 0.7, an Agrobacterium infection for 120 min, an Agrobacterium infective suspension at a pH of 5.0, an acetosyringone concentration of 200 µM, a cocultivation at 28 °C, a cocultivation for 72 h and a sucrose concentration of 30 g/L in the cocultivation medium. We also showed that preculture of wounded leaf explants for two days increased the regeneration rate. The integration of the desired gene into transgenic poplars was detected using selective medium containing kanamycin, followed by southern blot analysis. The expression of the transgene in the transgenic lines was confirmed by northern blot analysis.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24933641</PMID>
<DateCompleted><Year>2015</Year>
<Month>01</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>15</Volume>
<Issue>6</Issue>
<PubDate><Year>2014</Year>
<Month>Jun</Month>
<Day>13</Day>
</PubDate>
</JournalIssue>
<Title>International journal of molecular sciences</Title>
<ISOAbbreviation>Int J Mol Sci</ISOAbbreviation>
</Journal>
<ArticleTitle>An efficient Agrobacterium-mediated transformation system for poplar.</ArticleTitle>
<Pagination><MedlinePgn>10780-93</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms150610780</ELocationID>
<Abstract><AbstractText>Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybrid clone "Nanlin895" (Populus deltoides×P. euramericana) with Agrobacterium harboring the PBI121:CarNAC6 binary vector, we showed that the transformation efficiency was improved significantly by multiple independent factors, including an Agrobacterium infective suspension with an OD600 of 0.7, an Agrobacterium infection for 120 min, an Agrobacterium infective suspension at a pH of 5.0, an acetosyringone concentration of 200 µM, a cocultivation at 28 °C, a cocultivation for 72 h and a sucrose concentration of 30 g/L in the cocultivation medium. We also showed that preculture of wounded leaf explants for two days increased the regeneration rate. The integration of the desired gene into transgenic poplars was detected using selective medium containing kanamycin, followed by southern blot analysis. The expression of the transgene in the transgenic lines was confirmed by northern blot analysis.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Movahedi</LastName>
<ForeName>Ali</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. ali664699@gmail.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jiaxin</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. zhangjiaxin60633@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Amirian</LastName>
<ForeName>Rasoul</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Genomics, Agricultural Biotechnology Research Institute, Central Region of Iran (ABRICI), Najafabad Road, Isfahan 85135-487, Iran. rasool_amirian@yahoo.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhuge</LastName>
<ForeName>Qiang</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. qzhuge@njfu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>06</Month>
<Day>13</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Int J Mol Sci</MedlineTA>
<NlmUniqueID>101092791</NlmUniqueID>
<ISSNLinking>1422-0067</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012097">Repressor Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029863" MajorTopicYN="N">Cicer</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012097" MajorTopicYN="N">Repressor Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>03</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2014</Year>
<Month>06</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2014</Year>
<Month>06</Month>
<Day>04</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>6</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>6</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>1</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">24933641</ArticleId>
<ArticleId IdType="pii">ijms150610780</ArticleId>
<ArticleId IdType="doi">10.3390/ijms150610780</ArticleId>
<ArticleId IdType="pmc">PMC4100180</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Plant Physiol. 2009 Nov 15;166(17):1934-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19595478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2009 Mar;57(5):883-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18980645</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 1978 Jul 11;163(2):181-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">355847</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2002 Aug;14(8):1675-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12172015</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2013 Jan 25;14(2):2515-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23354481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Dec;133(4):1755-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14645724</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 Jan;149(1):88-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19126699</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2006;57:781-803</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16669782</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2007 Nov;65(4):357-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17929174</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2005;286:3-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15310910</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 1994 Feb;13(5):256-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24193760</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Nov;133(3):1011-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14551323</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2012 Feb;1819(2):97-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22037288</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1985 Mar 8;227(4691):1229-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17757866</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2010 Jan;29(1):97-110</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19956955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2010 Mar;29(3):211-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20087597</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Iran</li>
<li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name>
</noRegion>
<name sortKey="Zhang, Jiaxin" sort="Zhang, Jiaxin" uniqKey="Zhang J" first="Jiaxin" last="Zhang">Jiaxin Zhang</name>
<name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name>
</country>
<country name="Iran"><noRegion><name sortKey="Amirian, Rasoul" sort="Amirian, Rasoul" uniqKey="Amirian R" first="Rasoul" last="Amirian">Rasoul Amirian</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002376 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002376 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:24933641
   |texte=   An efficient Agrobacterium-mediated transformation system for poplar.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:24933641" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

An efficient Agrobacterium-mediated transformation system for poplar.

An efficient Agrobacterium-mediated transformation system for poplar.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki